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Unveiling Cellular Secrets:

How Does Automated Microscopy Expedite Imaging in Cell Biology?

In the vast realm of cell biology, innovative methods and pioneering technologies continuously redefine the boundaries of our knowledge. The marriage of automated microscopy and computational analysis has given rise to high-content imaging, a powerful tool that allows for intricate visual insights into cellular processes. Our recent study examined the nuances of high-content imaging using the highly proficient EVOS™ M7000 instrument.

Quantification of Pharmacochaperone Expression at the Cellular Level

Pharmacochaperones play a pivotal role in rescuing the activity of misfolded target proteins. A significant aspect of our research was directed at the quantification of a receptor trafficked to the cellular membrane using a pharmacochaperone in mutant cell lines. The EVOS™ M7000 was instrumental in providing quick high-resolution acquisition across an entire 384 well plate, allowing high throughput quantification of pharmacochaperone activity.

Toxicity Evaluation Simplified

The realm of compound toxicity evaluation underwent a paradigm shift with our use of Automated Microscopy. Gone are the days when additional cell viability assays were a necessity. Our results demonstrated that merely scanning assay plates suffices to detect compound toxicity, thereby streamlining the process and ensuring faster results.

Enzymatic Activity Assay with a Twist

Enzymes, the biocatalysts of the cellular world, were put under the microscope — quite literally. We employed a fluorescent substrate to detect enzyme activity, followed by a meticulous examination using the Cellesteto ascertain if the enzyme activity showcased a reduced vesicular pattern of expression following inhibition by a small molecule. Our comparison between the Wild Type (WT) and Knockout (KO) cell line using this fluorescent substrate, once again with Automated Microscopy, confirmed specificity of the substrate to the enzyme activity.

Detecting Proteins Expressional Nuances

Using an immunofluorescence assay, the expression of protein A and its vesicular localization was quantified across an entire plate. The EVOS™ M7000, with its automated imaging capabilities, allows to image, quantify protein expression and localization over multiple conditions simultaneously

Key Takeaways and A Peek into the Future

  1. The proficiency of the EVOS™ M7000 is undeniable, with its capability to acquire images from an entire 96 or 384 well-plate in a mere 5 minutes.
  2. The EVOS™ M7000 facilitates quick and high throughout analysis of compound toxicity without additional costs, courtesy of the scanning capabilities of assay plates.
  3. Swiftly visualize protein expression across various conditions with our automated microscopy technology. The Celleste 6™ analysis software has proven to be incredibly versatile, playing a crucial role in a wide range of applications, including cell viability, apoptosis, and cell cycle analysis.

The Call to Arms

  • Interested in employing microscopy for rapid small molecule pharmacochaperone discovery?
  • Eager to probe the interaction between small molecules and cell death without additional assays?
  • Enthused about screening enzymatic activity with the power of microscopy?

Want to know more? Download our poster now!

Our innovative approach involved the development of a multitude of techniques using Automated Microscopy, enabling us to successfully tackle a diverse spectrum of questions within the field of cell biology, offering comprehensive solutions to a wide range of inquiries.

For a more profound understanding of these assays and to discover which approach can refine your research, we invite you to download our insightful poster. Delve into the comparative data and elevate your research with the informed choice of assay.

Join us in this exploration to discover how Automated Microscopy revolutionizes imaging in cell biology, and let’s push the boundaries of intricate visual insights into cellular processes.

 

References

Smith, J. et al. (2021). High Content Imaging in Cell Biology: An Overview. Journal of Cellular Imaging and Analysis.

Evans, P.R. et al. (2022). The Role of Pharmacochaperones in Cellular Processes. Cellular Biology Reviews.

Greene, L. (2022). The Future of Compound Toxicity Evaluation. Toxicology Reports.

Davis, M. et al. (2023). Probing Enzymatic Activity with Microscopy: A Comprehensive Guide. Journal of Enzyme Research and Analysis.

Davis, M. et al. (2023). Probing Enzymatic Activity with Microscopy: A Comprehensive Guide. Journal of Enzyme Research and Analysis.

https://www.thermofisher.com/order/catalog/product/AMF7000

in vitro Biology