Case A – Solubility barrier

A compound’s solubility prevented HPLC analysis. We used a DCM/MeOH mix and transitioned to analytical SFC (UPC²).

• Excellent peak shape
• Retention time adapted for preparative scale
• Seamless handoff to prep-SFC

Case B – Apolar compound tailing in RP-HPLC

The compound produced tailing peaks under reverse-phase HPLC. Switched to achiral SFC, improving both separation and speed.

From Discovery to Preclinical—and Beyond

For our clients, the benefits of Sygnature’s separation sciences platform are clear:

• Speed: High-purity materials generated faster, even at scale
• Sustainability: Less solvent use, greener workflows
• Cost efficiency: No unnecessary GMP-grade overengineering in early phases
• Risk reduction: In-house method development and continuity through scale-up
• Clinical foresight: Prep methods transferable to CDMOs and GLP/GMP partners

We’re not just solving analytical puzzles, we’re preparing you for what’s next, with data and material you can act on.