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IN VITRO BIOLOGY

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SERVICES

in vitro Biology

Biochemistry and cell biology working together to answer key questions and go Beyond the Kit™.

Biochemistry & Enzymology

Cell Biology

Structural Biology

Need a robust assay?

Decisions are based on screening data. Primary, on target, in vitro screen data THAT YOU CAN TRUST is the most important element you need for your program. Working with a trusted partner is paramount for project success.

Reliability

Data you can trust

Reliable data is essential for decision-making, and the most important element you need for your program. We have identified multiple flaws in more than 30% of the assays being run by other groups. With our team, you’ll have primary, on target in vitro screening data that you can trust. Partnering with NuChem gives you the skill and reliability needed for project success.

Adaptability

Cutting-edge services

Take your projects to the next level with our in vitro biology capabilities. The cutting-edge services you can access with our team include developing assays & biomarkers, screening, expert consulting, biochemical & cellular assays and ADME-PK. Our capabilities are constantly evolving and provide flexibility for your specific needs.

Expertise

An unparalleled knowledge base

Add brain power to your projects with the extensive in vitro biology experience the NuChem team of scientists offers. You’ll benefit from our accomplishments in enzymology, protein production, assay development & miniaturization, target engagement, crystallography, and cellular MOA. Working collaboratively with NuChem adds instant expertise to your team.

Collaboration

Data you can trust

Getting the maximum value out of collaborative work requires a certain mindset. With our team, you benefit from our ability to manage communication and logistics for project success. When working collaboratively, our team shares knowledge so our experience becomes your advantage. Give your in vitro biology projects expert guidance through the development process with NuChem as your trusted partner.

Facilities

  • Multiple PCR and QPCR machines
  • Robot pipetting stations
  • LC-MS/MS
  • Multimode plate readers
  • Bright field and fluorescent microscopes
  • Western blot imager (in-cell & in-gel)
  • Capillary electrophoresis (Protein Simple system)
  • Cell culture facility (BSL2)
  • Biology and chemistry all under the same roof
  • Biophysics Instrumentation

Biochemical Assays

Enzyme Assays
  • Recombinant, whole cell, cell lysate
  • Target classes: oxidoreductases, transferases, hydrolases, lyases, isomerases, ligases
  • 384-well format
Detection Assays
  • ADP-Glo™
  • AlphaScreen™
  • LanthaScreen™
  • LCMS-MS
  • ELISA
Mechanism of Action
  • Enzyme kinetic parameters
  • Type of Inhibition/Activation (Active site, Allosteric)
  • Mechanism profiling to understand SAR
Protein-Protein Interaction
  • Competitive inhibition & binding assays
  • SPR (protein-protein interaction)
  • ITC
  • TSA
Protein-Inhibitor Binding
  • Measure binding kinetics, thermodynamics
  • NanoDSF
  • TSA
  • ITC

Cellular Assays

Primary Cells/Tissues
  • Primary neuronal cell culture from mouse & rat brain​
  • Primary splenocyte culture ​
  • Primary peripheral blood mononuclear cells (PBMCs)​
  • Monocyte, macrophage, and microglia primary cultures
  • And many more…
Established Cell lines (Human and animal cell lines)
Cell Line Development
Microscopy & Cell Imaging
  • Phase-contrast (bright field) microscopy
  • Fluorescent microscopy
  • ImageJ analysis software
Assay Readouts
  • Cell Visibility & Death
  • Reporter Assays
  • AlphsLISA
  • HTRF, BRET assays
  • Protein post-translational modifications
  • DNA Methylation

Protein & Gene Expression
  • Reporter gene assays​
  • Western blot​
  • Capillary electrophoresis (Protein Simple Inc. system)
  • PCR​ & QPCR
  • ELISA​, MSD
  • FACS
Assays Toward Different Target Classes
  • GPCRs (cAMP, calcium mobilization)​
  • PDEs​
  • Kinases​
  • Proteases
  • tRNA transferases
  • and others…

Structural Biology

Crystallography and protein production are key to understanding how drugs interact with proteins. The basis of our Target Insight™ approach. With the acquisition of IniXIum, NuChem now offers these key services:
  • Target production and characterization
  • Gene to structure projects
  • Crystallographic fragment screening
  • Protein production and crystallization
  • Cryo-EM
  • Structure determination of complexes
  • Discover more by visiting IniXium website.

Expert Consulting

  • Strategic biology planning for preclinical development
  • Expert unbiased reviews of current discovery projects
  • Problem-solving and troubleshooting of issues in screening and assay development
  • Oversight and management of outsourcing to other CROs
  • Due diligence and asset evaluation

Screening

  • Assay internalization & adaption from other CROs, institutes, or your own labs
  • Customized assays – we make it and run it
  • Up to 384-well format for enzyme- and cell-based assays
  • Robotic automation for liquid handling (screening libraries in 384-well format)

Biomarker Development

  • Testing services to support the unique needs of any clinical development program
  • Metabolic biomarkers with LC-MS/MS ​
  • Related biomarkers in body fluid or tissues
  • Access to FACS and MSD platform