NuChem is now part of the Sygnature Discovery Group. This signifies our continued growth and global integration but won’t affect our collaboration or the services you trust. Expect the same dedication and innovative solutions.

SERVICES

Protein & Structure

Protein Production

With over 60 protein scientists at Sygnature Discovery worldwide, we take great pride in providing high quality fully characterized proteins for a wide range of applications: from enzymatic assays to crystallography and Cryo-EM.

Expression Vector Preparation

  • Structure-guided sequence design
  • In-house design and subcloning for rapid turn-around of expression vector preparation/mutagenesis
  • Versatile expression vectors for wide choice of tagging for solubility enhancement, purification and downstream processing with tag cleavage possibility

Protein Production

  • Commercial and in-house modified E. coli strains for well folded proteins
  • Mammalian cell expression in HEK293-6E, CHO-3E7 and HEK Expi293
  • Insect cell expression in Sf9, Sf21, Hi5 cells
  • Expression screening of different conditions to optimize yield of well folded protein
  • Scale-up expression in the best condition up to 25L scale
  • 15N, 13C, 2H protein labeling to support NMR
  • Standard chromatography methods for purification of crystallography grade protein
  • In vivo biotinylation of target protein
  • Membrane protein production
  • Protein refolding from inclusion bodies
  • Low-level endotoxin batches