in vitro Biology
Biochemistry and cell biology working together to answer key questions and go Beyond the Kit™.
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Cell Apoptosis and Viabiity Assays
LPS-indueced cytokines released PK-PD study in mice serum
Need a robust assay?
Reliability
Data you can trust
Reliable data is essential for decision-making, and the most important element you need for your program. We have identified multiple flaws in more than 30% of the assays being run by other groups. With our team, you’ll have primary, on target in vitro screening data that you can trust. Partnering with NuChem gives you the skill and reliability needed for project success.
Adaptability
Cutting-edge services
Take your projects to the next level with our in vitro biology capabilities. The cutting-edge services you can access with our team include developing assays & biomarkers, screening, expert consulting, biochemical & cellular assays and ADME-PK. Our capabilities are constantly evolving and provide flexibility for your specific needs.
Expertise
An unparalleled knowledge base
Add brain power to your projects with the extensive in vitro biology experience the NuChem team of scientists offers. You’ll benefit from our accomplishments in enzymology, protein production, assay development & miniaturization, target engagement, crystallography, and cellular MOA. Working collaboratively with NuChem adds instant expertise to your team.
Collaboration
Data you can trust
Getting the maximum value out of collaborative work requires a certain mindset. With our team, you benefit from our ability to manage communication and logistics for project success. When working collaboratively, our team shares knowledge so our experience becomes your advantage. Give your in vitro biology projects expert guidance through the development process with NuChem as your trusted partner.
Facilities
- Multiple PCR and QPCR machines
- Robot pipetting stations
- LC-MS/MS
- Multimode plate readers
- Bright field and fluorescent microscopes
- Western blot imager (in-cell & in-gel)
- Capillary electrophoresis (Protein Simple system)
- Cell culture facility (BSL2)
- Biology and chemistry all under the same roof
- Biophysics Instrumentation
Biochemical Assays
Enzyme Assays
- Recombinant, whole cell, cell lysate
- Target classes: oxidoreductases, transferases, hydrolases, lyases, isomerases, ligases
- 384-well format
- How Do You Build Up a Higher-Throughput Enzymatic Assay?
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Detection Assays
- ADP-Glo™
- AlphaScreen™
- LanthaScreen™
- LCMS-MS
- ELISA
Mechanism of Action
- Enzyme kinetic parameters
- Type of Inhibition/Activation (Active site, Allosteric)
- Mechanism profiling to understand SAR
Protein-Protein Interaction
- Competitive inhibition & binding assays
- SPR (protein-protein interaction)
- ITC
- TSA
Protein-Inhibitor Binding
- Measure binding kinetics, thermodynamics
- NanoDSF
- TSA
- ITC
Cellular Assays
Primary Cells/Tissues
- Primary neuronal cell culture from mouse & rat brain
- Primary splenocyte culture
- Primary peripheral blood mononuclear cells (PBMCs)
- Monocyte, macrophage, and microglia primary cultures
- And many more…
Established Cell lines (Human and animal cell lines)
Cell Line Development
Microscopy & Cell Imaging
- Phase-contrast (bright field) microscopy
- Fluorescent microscopy
- ImageJ analysis software
Assay Readouts
- Cell Visibility & Death
Cell based assays for drug discovery: drug toxicity assessment.
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- Reporter Assays
- AlphsLISA
- HTRF, BRET assays
- Protein post-translational modifications
- DNA Methylation
Protein & Gene Expression
- Reporter gene assays
- Western blot
- Capillary electrophoresis (Protein Simple Inc. system)
How Is Capillary Electrophoresis Improving Protein Glycosylation Analysis?
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- PCR & QPCR
- ELISA, MSD
What Happens to Cytokine Levels When Inflammation Strikes?
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- FACS
Assays Toward Different Target Classes
- GPCRs (cAMP, calcium mobilization)
- PDEs
- Kinases
- Proteases
- tRNA transferases
- and others…
Structural Biology
- Target production and characterization
- Gene to structure projects
- Crystallographic fragment screening
- Protein production and crystallization
- Cryo-EM
- Structure determination of complexes
- Discover more by visiting IniXium website.
Expert Consulting
- Strategic biology planning for preclinical development
- Expert unbiased reviews of current discovery projects
- Problem-solving and troubleshooting of issues in screening and assay development
- Oversight and management of outsourcing to other CROs
- Due diligence and asset evaluation
Screening
- Assay internalization & adaption from other CROs, institutes, or your own labs
- Customized assays – we make it and run it
- Up to 384-well format for enzyme- and cell-based assays
- Robotic automation for liquid handling (screening libraries in 384-well format)
Biomarker Development
- Testing services to support the unique needs of any clinical development program
- Metabolic biomarkers with LC-MS/MS
- Related biomarkers in body fluid or tissues
- Access to FACS and MSD platform