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 Seeking Reliable Assays for Accurate Decision-Making? 

At the heart of successful drug discovery lies the foundation of dependable screening data. Here at NuChem Sciences, we understand the critical importance of robust, on-target, in vitro screening data that you can rely on. Trust is not just a word for us; it’s a commitment to providing you with the most reliable and accurate assays essential for your program’s success. 

Reliability in Data Accuracy

At NuChem Sciences, we prioritise the reliability of data above all. Understanding that accurate data is the cornerstone of effective decision-making in any program, we meticulously ensure the integrity of our in vitro screening data. Our rigorous quality checks have revealed flaws in over 30% of assays conducted elsewhere, highlighting our commitment to precision and trustworthiness. By choosing NuChem, you gain a partner dedicated to providing skilful, reliable data, setting the foundation for the success of your projects.

Adaptability with Advanced in vitro Biology Services

 Elevate your projects using NuChem Sciences’ state-of-the-art in vitro biology services. Our team offers a dynamic range of solutions, including assay and biomarker development, comprehensive screening, expert consulting, and specialised biochemical and cellular assays, along with ADME-PK studies. We pride ourselves on our evolving capabilities, tailored to meet the unique demands of your project with utmost flexibility and innovation.

Expertise

Leveraging a rich knowledge base in in vitro Biology

Enhance your projects with the profound expertise of NuChem Sciences’ team, bringing a wealth of experience in in vitro biology. Benefit from our extensive achievements in enzymology, protein production, advanced assay development and miniaturisation, precise target engagement, crystallography, and cellular mechanisms of action. Collaborating with NuChem means infusing your projects with deep, specialized knowledge and instant scientific expertise.

Collaboration

Enhancing outcomes with trusted data

Effective collaboration in scientific endeavours goes beyond mere teamwork; it demands a mindset geared towards excellence. At NuChem Sciences, we excel in orchestrating seamless communication and logistics, ensuring the success of your projects. Our collaborative approach is not just about working together; it’s about sharing our extensive knowledge and experience to turn them into your strategic advantage. Entrust your in vitro biology projects to NuChem and experience expert guidance every step of the way, solidifying us as your partner in innovation and trust.

State-of-the-Art Facilities: Equipped for Advanced Research

NuChem Sciences takes pride in its cutting-edge facilities, designed to cater to the diverse needs of modern scientific research. Our labs are equipped with:

  • Advanced PCR and QPCR machines for precise genetic analysis.
  • Automated robot pipetting stations for efficient and accurate sample handling.
  • High-performance LC-MS/MS systems for detailed mass spectrometry.
  • Versatile multimode plate readers for a range of assay types.
  • High-resolution bright field and fluorescent microscopes for intricate cellular studies.
  • Sophisticated Western blot imager for both in-cell and in-gel applications.
  • State-of-the-art capillary electrophoresis (Protein Simple system) for precise protein analysis.
  • A fully equipped cell culture facility (BSL2) ensuring optimal cell growth conditions.
  • Advanced biophysics instrumentation for detailed molecular studies.

Each aspect of our facility is designed to enhance research capabilities, ensuring that every project benefits from the latest technological advancements in both biology and chemistry.

Biochemical Assays

Enzyme Assays

  • Recombinant, whole cell, cell lysate
  • Target classes: oxidoreductases, transferases, hydrolases, lyases, isomerases, ligases
  • 384-well format
  • How Do You Build Up a Higher-Throughput Enzymatic Assay?
    READ OUR BLOG
    DOWNLOAD OUR POSTER

Detection Assays

  • ADP-Glo™
  • AlphaScreen™
  • LanthaScreen™
  • LCMS-MS
  • ELISA

Mechanism of Action

  • Enzyme kinetic parameters
  • Type of Inhibition/Activation (Active site, Allosteric)
  • Mechanism profiling to understand SAR

Protein-Protein Interaction

  • Competitive inhibition & binding assays
  • SPR (protein-protein interaction)
  • ITC
  • TSA

Protein-Inhibitor Binding

  • Measure binding kinetics, thermodynamics
  • NanoDSF SPR
  • TSA
  • ITC

At NuChem Sciences, our biochemical assays are designed for precision, versatility, and high-throughput capabilities, ensuring that your research is supported by the most accurate and reliable data.

Cellular Assays

Assay Readouts

  • Reporter Assays
  • AlphaLISA
  • HTRF, BRET assays
  • Protein post-transitional modifications
  • DNA Methylation
  • Cell Viability & Death
    Cell based assays for drug discovery: drug toxicity assessment
    READ OUR BLOG
    DOWNLOAD OUR POSTER

Protein & Gene Expression

Assays Toward Different Target Classes

  • GPCRs (cAMP, calcium mobilization)​
  • PDEs​
  • Kinases​
  • Proteases
  • tRNA transferases
  • and others…

Primary Cells/Tissues

  • Primary neuronal cell culture from mouse & rat brain​
  • Primary splenocyte culture ​
  • Primary peripheral blood mononuclear cells (PBMCs)​
  • Monocyte, macrophage, and microglia primary cultures
    Can iPSC-Derived Microglia Hold the Key to Unlocking Neurodegenerative Mysteries?
    READ OUR BLOG
    DOWNLOAD OUR POSTER
  • And many more…

Established Cell lines (Human and animal cell lines)

Cell Line Development Microscopy & Cell Imaging

  • Phase-contrast (bright field) microscopy​
  • Fluorescent microscopy
    How Does Automated Microscopy Expedite Imaging in Cell Biology?
    READ OUR BLOG
    DOWNLOAD OUR POSTER
  • ImageJ analysis software

Structural Biology

Crystallography and protein production are key to understanding how drugs interact with proteins. The basis of our Target Insight™ approach. With the acquisition of IniXIum, NuChem now offers these key services:
  • Target production and characterization
  • Gene to structure projects
  • Crystallographic fragment screening
  • Protein production and crystallization
  • Cryo-EM
  • Structure determination of complexes
  • Discover more by visiting IniXium website.

Expert Consulting

  • Strategic biology planning for preclinical development
  • Expert unbiased reviews of current discovery projects
  • Problem-solving and troubleshooting of issues in screening and assay development
  • Oversight and management of outsourcing to other CROs
  • Due diligence and asset evaluation

Screening

  • Assay internalization & adaption from other CROs, institutes, or your own labs
  • Customized assays – we make it and run it
  • Up to 384-well format for enzyme- and cell-based assays
  • Robotic automation for liquid handling (screening libraries in 384-well format)

Biomarker Development

  • Testing services to support the unique needs of any clinical development program
  • Metabolic biomarkers with LC-MS/MS ​
  • Related biomarkers in body fluid or tissues
  • Access to FACS and MSD platform